PNC27*5mg 5ml

PNC-27 is a chimeric peptide construct designed to target the HDM-2 (human double minute 2) oncoprotein, which is overexpressed on the plasma membrane of various cancer cell types but minimally present in normal untransformed cells . With a molecular weight of approximately 4031.7 Daltons and the molecular formula C188H293N53O44S, this 32-amino acid peptide consists of two functional domains: an HDM-2-binding sequence corresponding to residues 12-26 of the p53 tumor suppressor protein, fused to a transmembrane-penetrating leader sequence (penetratin) derived from the Drosophila antennapedia homeodomain . Structural studies confirm that the three-dimensional conformation of the p53 residues within PNC-27 is superimposable on the structure of the same residues when bound to HDM-2, suggesting this conformation is critical for target recognition and binding .

The primary mechanism of action in research models involves PNC-27 binding specifically to HDM-2 expressed on cancer cell membranes, triggering the formation of cytotoxic transmembrane pores . This pore formation disrupts membrane integrity, leading to rapid ion flux, osmotic imbalance, and necrotic cell death characterized by lactate dehydrogenase (LDH) release, typically observed within hours of exposure . Notably, this pathway operates independently of p53 transcriptional activity, as PNC-27 has demonstrated efficacy against p53-null and p53-mutated cancer cell lines including acute myelogenous leukemia and pancreatic cancer models . In contrast, untransformed primary cells such as fibroblasts and hematopoietic cells show minimal susceptibility, attributable to negligible HDM-2 membrane expression .

This research-grade product is supplied as a sterile, white lyophilized powder with purity ≥97% as determined by RP-HPLC analysis . For experimental use, reconstitution is recommended in sterile 18MΩ-cm H2O at concentrations not less than 100 µg/mL, with further dilution in appropriate aqueous buffers as required by specific protocols . The product is intended strictly for laboratory research applications and is not approved for human or veterinary therapeutic use. For long-term storage, lyophilized powder should be kept desiccated below -18°C; reconstituted solutions may be stored at 4°C for 2-7 days, with carrier protein (0.1% HSA or BSA) recommended for extended storage . Avoid repeated freeze-thaw cycles to preserve peptide integrity.